[现货 现代液相色谱技术导论 第3版 英文原版 Introduction To Modern Liquid Chromatography Lloyd Snyder 化学【中商原版】]
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[Introduction To Modern Liquid Chromatography, 3rd Edition]
[基本信息]
Hardcover: 960 pages
Publisher: Wiley-Blackwell; 3rd edition (12 Jan. 2010)
Language: English
ISBN-10: 0470167548
ISBN-13: 978-0470167540
[Product Dimensions: 186 x 265 x 55mm | 2,078g]
[页面参数仅供参考，具体以实物为准]
[书籍简介]
[高效液相色谱法（HPLC）是当今化学分析及其相关领域的领先技术，它可用于分离、分析和（或）纯化几乎所有的样品。Snyder和Kirkland的这本《现代液相色谱技术导论》，一直以来就是关于HPLC技术的重要专著。]
[《现代液相色谱技术导论（第3版）》反映了当前][最新][的研究成果及实践经验，以引导读者认识HPLC，了解它与其他现代分离技术的关系以及它的历史为开端，逐步引领读者深入了解以下专题：]
- [HPLC分离的基础及不同实验条件的常规影响]
- [仪器和检测]
- [色谱柱-HPLC系统的“心脏”]
- [反相分离，正相色谱，梯度洗脱，二维分离和其他技术]
- [计算机模拟，定量和定性分析，方法验证及质量控制]
- [大分子（包括生物大分子和合成聚合物）的分析]
- [手性分离，制备分离和样品配备]
- [如何系统地建立HPLC的分离方法]
- [故障检修的技15，技术以及仪器和色谱故障的实例分析]
[《现代液相色谱技术导论》旨在满足不同读者的需求，无论你是初学者还是专家，本书为你提供了][最新、最全面][以及通俗易懂的HPLC方法及其应用方面的知识。]
[The latest edition of the authoritative reference to HPLC]
[High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve pro]blems that were troubl[esome in the past, and the application of HPLC for new kinds of samples.]
[This carefully considered Third Edition maintains the strengths of the previous edition while significantly modifying its organization in light of recent research and experience. The text begin]s b[y introducing the reader to HPLC, its use in relation to other modern separation techniques, and its history, then leads into such specific topics as:]
- [The basis of HPLC separation and the general effects of different experimental conditions]
- Equipment and detection
- [The column—the ]"heart" of the HPLC system
- [Reversed-phase separation, normal-phase chromatography, gradient elution], tw[o-dimensional separation, and other techniques]
- [Computer simulation, qualitative and quantitative analysis, and method validation and quality control]
- [The separation of large molecules, including both biological and synthetic polymers]
- [Chiral separations, preparative separations, and sample preparation]
- [Systematic development of HPLC separations—new to this edition]
- Troubl[eshooting tricks, techniques, and case studies for both equipment and chromatograms]
[Designed to fulfill the needs of the full range of HPLC users, from novices to experts, Introduction to Modern Liquid Chromatography, Third Edition offers the most up-to-date, comprehensive, and accessi]bl[e survey of HPLC methods and applications availa]ble.
[目录]
PREFACE.
GLOSSARY OF SYMBOLS AND ABBREVIATIONS.
1 INTRODUCTION.
1.1 Background Information.
1.2 A Short History of HPLC.
1.3 Some Alternatives to HPLC.
1.4 Other Sources of HPLC Information.
References.
[2 BASIC CONCEPTS AND THE CONTROL OF SEPARATION.]
2.1 Introduction.
2.2 The Chromatographic Process.
2.3 Retention.
[2.4 Peak Width and the Column Plate Number N.]
2.5 Resolution and Method Development.
2.6 Sample Size Effects.
2.7 RELATED TOPICS.
References.
3 EQUIPMENT.
3.1 Introduction.
3.2 Reservoirs and Solvent Filtration.
3.3 Mobile-Phase Degassing.
3.4 Tubing and Fittings.
3.5 Pumping Systems.
3.6 Autosamplers.
3.7 Column Ovens.
3.8 Data Systems.
3.9 Extra-Column Effects.
3.10 Maintenance.
References.
4 DETECTION.
4.1 Introduction.
4.2 Detector Characteristics.
4.3 Introduction to Individual Detectors.
4.4 UV-Visible Detectors.
4.5 Fluorescence Detectors.
4.6 Electrochemical (Amperometric) Detectors.
4.7 Radioactivity Detectors.
4.8 Conductivity Detectors.
4.9 Chemiluminescent Nitrogen Detector.
4.10 Chiral Detectors.
4.11 Refractive Index Detectors.
4.12 Light-Scattering Detectors.
4.13 Corona-Discharge Detector (CAD).
4.14 Mass Spectral Detectors (MS).
4.15 Other Hyphenated Detectors.
4.16 Sample Derivatization and Reaction Detectors.
References.
5 THE COLUMN.
5.1 Introduction.
5.2 Column Supports.
5.3 Stationary Phases.
5.4 Column Selectivity.
5.5 Column Hardware.
5.6 Column-Packing Methods.
5.7 Column Specifications.
5.8 Column Handling.
References.
[6 REVERSED-PHASE CHROMATOGRAPHY FOR NEUTRAL SAMPLES.]
6.1 Introduction.
6.2 Retention.
6.3 Selectivity.
[6.4 Method Development and Strategies for Optimizing Selectivity.]
[6.5 Nonaqueous Reversed-Phase Chromatography (NARP).]
6.6 Special Problems.
References.
[7 IONIC SAMPLES: REVERSED-PHASE, ION-PAIR, AND IONEXCHANGE CHROMATOGRAPHY.]
7.1 Introduction.
[7.2 Acid-Base Equilibria and Reversed-Phase Retention.]
7.3 Separation of Ionic Samples by Reversed-Phase Chromatography (RPC).
7.4 Ion-Pair Chromatography (IPC).
7.5 Ion-Exchange Chromatography (IEC).
References.
8 NORMAL-PHASE CHROMATOGRAPHY.
8.1 Introduction.
8.2 Retention.
8.3 Selectivity.
8.4 Method-Development Summary.
8.5 Problems in the Use of NPC.
[8.6 Hydrophilic Interaction Chromatography (HILIC).]
References.
9 GRADIENT ELUTION.
9.1 Introduction.
[9.2 Experimental Conditions and Their Effects on Separation.]
9.3 Method Development.
9.4 Large-Molecule Separations.
9.5 Other Separation Modes.
9.6 Problems.
References.
[10 COMPUTER-ASSISTED METHOD DEVELOPMENT.]
10.1 Introduction.
10.2 Computer-Simulation Software.
10.3 Other Method-Development Software.
[10.4 Computer Simulation and Method Development.]
References.
[11 QUALITATIVE AND QUANTITATIVE ANALYSIS.]
11.1 Introduction.
11.2 Signal Measurement.
11.3 Qualitative Analysis.
11.4 Quantitative Analysis.
11.5 Summary.
References.
12 METHOD VALIDATION.
12.1 Introduction.
12.2 Terms and Definitions.
12.3 System Suitability.
12.4 Documentation.
[12.5 Validation for Different Pharmaceutical-Method Types.]
12.6 Bioanalytical Methods.
12.7 Analytical Method Transfer (AMT).
[12.8 Method Adjustment or Method Modification.]
[12.9 Quality Control and Quality Assurance.]
12.10 Summary.
References.
[13 BIOCHEMICAL AND SYNTHETIC POLYMER SEPARATIONS.]
13.1 Biomacromolecules.
[13.2 Molecular Structure and Conformation.]
[13.3 Special Considerations for Biomolecule HPLC.]
[13.4 Separation of Peptides and Proteins.]
13.5 Separation of Nucleic Acids.
13.6 Separation of Carbohydrates.
13.7 Separation of Viruses.
[13.8 Size-Exclusion Chromatography (SEC).]
[13.9 Large-Scale Purification of Large Biomolecules.]
13.10 Synthetic Polymers.
References.
14 ENANTIOMER SEPARATIONS.
14.1 Introduction.
14.2 Background and Definitions.
14.3 Indirect Method.
14.4 Direct Method.
14.5 Peak Dispersion and Tailing.
[14.6 Chiral Stationary Phases and Their Characteristics.]
14.7 Thermodynamic Considerations.
References.
15 PREPARATIVE SEPARATIONS.
15.1 Introduction.
15.2 Equipment for Prep-LC Separation.
15.3 Isocratic Elution.
15.4 Severely Overloaded Separation.
15.5 Gradient Elution.
15.6 Production-Scale Separation.
References.
16 SAMPLE PREPARATION.
16.1 Introduction.
16.2 Types of Samples.
[16.3 Preliminary Processing of Solid and Semi-Solid Samples.]
[16.4 Sample Preparation for Liquid Samples.]
16.5 Liquid-Liquid Extraction.
16.6 Solid-Phase Extraction (SPE).
[16.7 Membrane Techniques in Sample Preparation.]
[16.8 Sample Preparation Methods for Solid Samples.]
16.9 Column-Switching.
[16.10 Sample Preparation for Biochromatography.]
16.11 Sample Preparation for LC-MS.
16.12 Derivatization in HPLC.
References.
17 TROUBLESHOOTING.
Quick Fix.
17.1 Introduction.
17.2 Prevention of Problems.
17.3 Problem-Isolation Strategies.
17.4 Common Symptoms of HPLC Problems.
17.5 Troubleshooting Tables.
References.
[APPENDIX I. PROPERTIES OF HPLC SOLVENTS.]
I.1 Solvent-Detector Compatibility.
I.1.1 UV Detection.
I.1.2 RI Detection.
I.1.3 MS Detection.
I.2 Solvent Polarity and Selectivity.
I.3 Solvent Safety.
References.
[APPENDIX II. PREPARING BUFFERED MOBILE PHASES.]
II.1 Sequence of Operations.
[II.2 Recipes for Some Commonly Used Buffers.]
Reference.
Index.
[作者简介]
[Lloyd R. Snyder, PhD, is a Principal at LC Resources in Walnut Creek, California. He is the author or coauthor of several books including An Introduction to Separation Science, Introduction to Modern Liquid Chromatography, Second Edition, the bestselling Practical HPLC Method Development, Second Edition, and the comprehensive High-Performance Gradient Elution, all pu]bl[ished by Wiley. Joseph J. Kirkland, PhD, is Vice President of Research and Development for Advanced Materials Technology, Inc., and coauthor of Introduction to Modern Liquid Chromatography, Second Edition, Practical HPLC Method Development, Second Edition, and Modern Size-Exclusion Liquid Chromatography, Second Edition, all pu]bl[ished by Wiley. John W. Dolan, PhD, is a Principal at LC Resources. He is author of the popular ]"LC Troubleshooting"[ column in LCGC magazine and coauthor with Lloyd Snyder of Trou]bl[eshooting LC Systems and High-Performance Gradient Elution.]
